TABLE 1.

Total recovery of Neu5Gc after feeding free Neu5Gc or Neu5Gc-glycoproteins (glycosidically bound Neu5Gc)

We gavaged equal amounts of Neu5Gc in the form of free Neu5Gc or Neu5Gc-glycoproteins into Cmah^−/− animals. At the indicated time points, we isolated tissues (stomach, intestines, liver, and blood) and intestinal contents (stomach, small intestine, and large intestine) and quantified the total Neu5Gc that could be recovered as a percentage of the total Neu5Gc fed (0.3 mmol_Neu5Gc). These recovery values omit any Neu5Gc in the urine (studied separately, see Fig. 1, E and F) due to the impracticality of consistent urine collection in all mice. No Neu5Gc was detected in fecal material.

Hours after feeding	Hours after feeding	2 h	4 h	6 h
% Neu5Gc recovered	Neu5Gc-glycoprotein fed	60.44 ± 4.8	5.99 ± 2.1	0.40
	Free Neu5Gc fed	18.37	1.50	<0.01