FIGURE 1.

DIM reverses anoikis resistance in ovarian cancer cells. Ai, A2780; Aii, SKOV-3; Aiii, TOV-21G; and Aiv, OVCAR-429 cells were grown in poly-HEMA-coated plates as suspension culture and treated with DMSO or various concentrations of DIM. After 24 h, cells were replated in an adherent 24-well plate and allowed to attach overnight. Viable cells were analyzed by Sulforhodamine B (SRB) assay. Representative bar graphs show the percentage anoikis resistance in different treatment conditions. *, p < 0.05 compared with control. Error bars, S.D. B, blots are representative of Gli1, Cl-caspase3, and Cl-PARP from lysates collected from A2780 (i) and OVCAR-429 cells (ii) grown under suspension culture conditions and treated with or without 50 μm DIM. Actin was used as loading control. C, representative blot of Gli1 and Cl-PARP from lysates collected from A2780 cells grown under suspension culture conditions and treated with 50 μm DIM for different time points are shown. Actin was used as a loading control. D, A2780 cells were grown for 24 h under adherent and suspension culture conditions. The cells were trypsinized and replated in a 24-well plate and allowed to attach overnight. Viable cells were analyzed by SRB assay. Representative bar graphs show the percentage anoikis resistance under different growth conditions. E, representative Western blots are shown of Gli1, Cl-caspase3, and Cl-PARP from lysates collected from A2780 cells grown under adherent and suspension culture conditions. F, representative blots of Gli1, Cl-caspase3, and Cl-PARP from lysates collected from A2780 cells grown under adherent culture conditions and treated with 50 μm DIM are shown. Actin was used as a loading control.