TABLE 1.
Theoretical and observed monoisotopic mass values for peptide fragments that included cysteine residues
Peptide fragments that included Cys266, Cys398, and Cys114 and a disulfide-linked peptide were obtained from EF-G that had been treated with 5 mm DTT (reducing) or 0.5 mm H2O2 (oxidizing). Peaks 1 through 3 correspond to those shown in supplemental Fig. S3.
| Peak 1 (Cys266) | Peak 2 (Cys398) | Peak 3 (Cys114) | Peak 4 (Cys114-Cys266) | |||||
|---|---|---|---|---|---|---|---|---|
| Expected | 1721.04 | Δa | 2044.26 | Δ | 2564.96 | Δ | 4283.01 | Δ |
| Reducing | 1721.58 | +0.54 | 2045.34 | +0.08 | 2565.20 | +0.06 | NDb | |
| Oxidizing | 1721.45 | +0.41 | 2045.31 | +0.05 | 2565.12 | +0.16 | 4282.49 | −0.52 |
a Δ, difference between given value and expected value.
b ND, not detected.