FIGURE 5.

Effects of phosphorylation of MACFp1 on EB1 binding. The sequence 2–28 of MACFp1 is shown at the top. The serine and threonine residues that were systematically phosphorylated are underlined, and the corresponding residue number is shown below. The SKIP motif is highlighted by a line above the sequence. The bottom panel displays schematic representations of the phosphorylated MACFp1 variants tested. Phosphorylated residues are indicated. For simplicity, double numbering of sites (e.g. pS5/8) refers to single modified residues (i.e. phospho-Ser-5 or -8 (pS5 or pS8)). Phosphorylated MACFp1 variants are classified as EB1 binders (right) and non-binders (left). See also supplemental Fig. 2.