TABLE 1.
Primary antibodies used for immunostaining
| Antigen | Company or source | Host | Dilution |
|---|---|---|---|
| E-cadherin | BD Transduction Laboratories | Mouse | 1:1,500 |
| β-Catenin | BD Transduction Laboratories | Mouse | 1:1,500 |
| Cytokeratin 19 | Tanimizu et al. (21) | Rabbit | 1:2,000 |
| EpCAM | BD Pharmingen | Rabbit | 1:500 |
| α6 integrin | Biolegend | Rat | 1:500 |
| β1 integrin | Chemicon | Rat | 1:500 |
| β4 integrin | BD Pharmingen | Rat | 1:500 |
| HNF4α | Santa Cruz Biotechnology | Goat | 1:200 |
| Laminin α1 | A gift from Dr. Takako Sasaki (Oregon Health and Science University) | Rabbit | 1:2,000 |
| Laminin α5 | A gift from Dr. Takako Sasaki (Oregon Health and Science University) | Rabbit | 1:2,000 |
| Protein kinase ζ | Santa Cruz Biotechnology | Rabbit | 1:100 |
| Sox9 | Millipore | Rabbit | 1:500 |
| ZO1 | Zymed Laboratories Inc. | Rabbit | 1:200 |
| ZO1 | A gift from Dr. Bruce Stevenson (University of Alberta) | Rat | 1:2,000 |