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. 2012 Jul 2;287(34):29159–29167. doi: 10.1074/jbc.M112.365049

FIGURE 3.

FIGURE 3.

Ability of estrogen to increase bone mass in wild-type mice requires functional OTRs. Two-month-old OTR−/− mice or wild-type (OTR+/+) littermates were treated with 17β-estradiol (E2; 50 μg/kg) or placebo biweekly (cumulative dose of 100 μg/kg/week). Representative images show the effect of 17β-estradiol on trabecular bone volume (A), double-labeled surfaces (B), and cortical thickness (C) in wild-type and OTR−/− mice. D, serum osteocalcin (by ELISA) 12 h after a single 17β-estradiol injection of 50 μg/kg. Statistics were as follows: Student's t test with the Bonferroni correction, comparing 17β-estradiol treatment versus zero dose (*, p < 0.05; **, p < 0.01), as well as wild-type versus OTR−/− mice (^^, p < 0.01).