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. 2012 Jun 21;287(34):29204–29212. doi: 10.1074/jbc.M112.371880

FIGURE 1.

FIGURE 1.

Synthetic pathway for Ser/Thr O-linked LacdiNAc-containing structures on core 2 oligosaccharides. O-Linked oligosaccharides with a core 2 structure containing a GlcNAc-linked β1,6 to the O-linked GalNAc (structure 4) can be further modified by the addition of either β1,4-linked Gal or GalNAc to generate structures 5 and 7, respectively. Structure 7 can be further modified to generate structures 8, 9, 10, and 11. CHO cells do not express the β1,6GlcNAc transferase C2GnT1 and are not able to synthesize core 2 O-linked structures. β1,3 GalT, β1,3Galactosyltransferase; β4GalNAc-T, β1,4-N-Acetylgalactosaminyltransferase; NeuNAcT, NeuNAc transferase.