FIGURE 6.

Cellobiose inhibition of TrCel7A on ¹⁴C-BC in “steady state.” A and B, release of ¹⁴CB in hydrolysis of ¹⁴C-BC by individual TrCel7A (A) or TrCel7A + EG (B). The concentration of ¹⁴C-BC and TrCel7A was 0.25 mg ml⁻¹ and 0.25 μm, respectively. If present, the concentration of EG (TrCel5A) was 0.025 μm. Series without added cellobiose were provided with 0.125 μm β-glucosidase (♢). Concentration of added cellobiose was 0 mm (♢), 0 mm without β-glucosidase (♦), 0.2 mm (*), 0.5 mm (△), 1.0 mm (+), 2.0 mm (○), 5.0 mm (×), or 10 mm (□). C and D, hydrolysis data were rearranged to obtain the ratio of ([¹⁴CB]_CB/[¹⁴CB]_{CB = 0}) for each time point. Subscripts CB and CB = 0 refer to the measurements in the presence and absence of cellobiose, respectively. Plotted are average values over all time points (except TrCel7A + EG) with corresponding error bars (S.D.). Solid lines are from the non-linear regression according to supplemental Equation S1 (individual enzymes) or Equation 9 (synergistic mixtures). C, TrCel7A (♢) and TrCel7A + EG (♦). D, CD_TrCel7A (△) and CD_TrCel7A + EG (▴).