TABLE 2.
DSE between CBH TrCel7A and EG TrCel5A in hydrolysis of BC
| BC |
TrCel7A and TrCel5Aa |
CDTrCel7A and TrCel5Aa |
||||
|---|---|---|---|---|---|---|
| DSEGlcb | DSEOAc | DSEkd | DSEGlcb | DSEOAc | DSEkd | |
| mg ml−1 | ||||||
| 0.1 | 23 ± 7.9 | 1.7 ± 0.70 | 14.5 ± 5.3 | |||
| 0.25 | 12 ± 1.6 | 1.5 ± 0.32 | 7.8 ± 2.4 | 5.3 ± 1.9 | 0.25 ± 1.30 | 12.5 ± 2.4 |
| 0.5 | 7.7 ± 0.8 | 1.3 ± 0.15 | 5.6 ± 1.2 | 4.8 ± 1.3 | 0.64 ± 0.33 | 5.9 ± 1.3 |
| 1.0 | 4.5 ± 0.5 | 1.1 ± 0.04 | 4.0 ± 1.3 | 4.4 ± 0.9 | 0.63 ± 0.03 | 5.2 ± 2.4 |
| 2.5 | 3.0 ± 0.7 | 1.1 ± 0.03 | 2.7 ± 1.0 | 2.6 ± 0.4 | 0.81 ± 0.14 | 2.6 ± 0.8 |
a Experimental conditions were as follows: [TrCel7A] or [CDTrCel7A] = 0.5 μm, [β-glucosidase] = 0.85 μm, 25 °C, pH 5.0. If present, the concentration of EG (TrCel5A) was 0.1 μm. Samples were withdrawn after 2, 5, and 10 min of hydrolysis and analyzed for glucose formation and [TrCel7A]OA.
b DSE on the level of glucose formation (DSEGlc) represents the ratio of glucose produced by the mixture of TrCel7A and TrCel5A to glucose produced by individual TrCel7A. Glucose formation after 10 min of hydrolysis was used in calculations of DSEGlc. Low levels of glucose produced by individual 0.1 μm TrCel5A were not accounted for in calculating DSEGlc.
c DSE on the level of [TrCel7A]OA (DSEOA) represents the ratio of [TrCel7A]OA measured for the mixture of TrCel7A and TrCel5A to [TrCel7A]OA measured for individual TrCel7A. For series with intact TrCel7A, an average value of [TrCel7A]OA taken over all time points was used in calculations of DSEOA, whereas for CDTrCel7A, a [CDTrCel7A]OA measured after 10 min of hydrolysis was used.
d DSE on the level of observed rate constant (kobs) (DSEk) represents the ratio of kobs measured for the mixture of TrCel7A and TrCel5A to kobs measured for individual TrCel7A. An average value of kobs taken over all time points was used in calculations of DSEk.