TABLE 3.

CB inhibition of TrCel7A in “steady state”

Enzyme(s)a	IC50 for CBb
	mm
TrCel7A	0.65 ± 0.15
TrCel7A + EG	0.38 ± 0.03
CDTrCel7A	0.72 ± 0.28
CDTrCel7A + EG	0.46 ± 0.07

^a Experiment conditions were as follows: [¹⁴C-BC] = 0.25 mg ml⁻¹, [TrCel7A] or [CD_TrCel7A] = 0.25 μm, 25 °C, pH 5.0. If present, the concentration of EG (TrCel5A) was 0.025 μm.

^b IC₅₀ for cellobiose was found from the data in Fig. 6, C and D, using non-linear regression according to supplemental Equation S1 (for individual TrCel7A or its CD) or Equation 9 (for synergistic mixture).