TABLE 3.
Enzyme(s)a | IC50 for CBb |
---|---|
mm | |
TrCel7A | 0.65 ± 0.15 |
TrCel7A + EG | 0.38 ± 0.03 |
CDTrCel7A | 0.72 ± 0.28 |
CDTrCel7A + EG | 0.46 ± 0.07 |
a Experiment conditions were as follows: [14C-BC] = 0.25 mg ml−1, [TrCel7A] or [CDTrCel7A] = 0.25 μm, 25 °C, pH 5.0. If present, the concentration of EG (TrCel5A) was 0.025 μm.
b IC50 for cellobiose was found from the data in Fig. 6, C and D, using non-linear regression according to supplemental Equation S1 (for individual TrCel7A or its CD) or Equation 9 (for synergistic mixture).