Skip to main content
. 2012 Jun 25;287(34):28656–28665. doi: 10.1074/jbc.M112.356584

FIGURE 3.

FIGURE 3.

VPA reduces periphery T cell population in EAE mice. A and B, naive and EAE mice were treated with VPA (300 mg/kg, orally, starting from day 3) or vehicle. The body weight (B) and spleen weight (A) were measured at day 10. The data are the means ± S.E. (n = 6). ***, p < 0.001, versus naive control; #, p < 0.05, versus vehicle control (Student's t test). C and D, leukocytes were isolated from the spleen (C) and circulating blood (D) of naive and EAE animals treated with VPA (300 mg/kg, orally, starting from day 3) or vehicle control at day 10 postimmunization, and the percentage and number of CD4+ T cells, CD8+ T cells, and B cells (B220+) were analyzed with surface staining and flow cytometry. TH-1 and TH-17 cells were analyzed by intracellular staining of IFN-γ and IL-17a in the CD4 gate. E–G, statistical analysis of C. H and I, statistical analysis of D. The data represent the means ± S.E. (n = 6). *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus naive control. #, p < 0.05; ##, p < 0.01; ###, p < 0.001 versus vehicle treatment group (Student's t test).