FIGURE 10.

NatB silencing does not affect the subcellular localization of endogenous or overexpressed α-syn in HEK cells. 24 h post-transfecting HEK cells with plasmids encoding scrambled shRNA or NatB shRNA4, cells were either transfected with a plasmid encoding WT α-syn (upper 2 panels) or not (lower 2 panels) and then immunostained using the Syn-1 antibody. Confocal fluorescent microscopy shows that cells transfected with scrambled or NatB shRNA4 constructs (expressing enhanced GFP) show similar subcellular localization of endogenously and exogenously expressed synuclein. Nuclei were counterstained with Hoechst 33342.