FIGURE 2.

RT-PCR analysis of different genotypes of GnT-V and GnT-Vb K/O mice. Total RNA was isolated from the brain tissues of GnT-V(+/+)Vb(+/+), GnT-V(−/+)Vb(−/+), GnT-V(−/−) Vb(+/+), GnT-V(+/+)Vb(−/−), and GnT-V(−/−)Vb(−/−) mice. Semi-quantitative, real time, one-step reverse transcription (RT)-PCR was performed to amplify a 319-bp GnT-Vb cDNA region containing exons 6 and 7 as described under “Experimental Procedures.” GnT-V expression was analyzed as described and showed a 119-bp PCR product. −RT, no reverse transcriptase reaction. G6PDH (glutaraldehyde-6-phosphate dehydrogenase) PCRs were used as control.