FIGURE 5.

Analysis of α-dystroglycan glycosylation and laminin overlay assay. Western blot and overlay analysis indicates that double deletion of GnT-V and GnT-Vb does not alter the reactivity of α-dystroglycan to the 116C4 carbohydrate-specific epitope or its ability to bind laminin. Wheat germ agglutinin-enriched samples from wild type (+/+, +/+) or GnT-V and -Vb double knock-out (−/−, −/−) brains were analyzed by Western blot and laminin overlay assays. Each two mice brain tissues were applied. The IIH6C4 antibody detects a specific carbohydrate epitope that is lost from α-dystroglycan in many forms of CMDs and animal models in which O-mannosylation is disrupted. It is, however, unaffected by the absence of GnT-Vb and -Va. The ability of α-dystroglycan to bind laminin seems similarly unaffected.