TABLE 2.
Specific N-linked oligosaccharides identified by MS/MS mass spectrometry in brain tissue of various genotypes
O indicates observed; X indicates below level of detection (<0.05 relative to wild type); dc indicates doubly charged; tc indicates triply charged; ( ), denotes triply charged species.
| No. | N-Linked oligosaccharide composition | m/z (mono), [M + Na]* | V(+/+) Vb(+/+), dc(tc) | V(−/+) Vb(−/+), dc(tc) | V(−/−) Vb(+/+),dc(tc) | V(+/+) Vb(−/−), dc(tc) | V(−/−) Vb(−/−), dc(tc) |
|---|---|---|---|---|---|---|---|
| 1 | (Gal)1(GlcNAc)5(Man)3(GlcNAc)2(Fuc)1 | 2775.405 | O | O | X | O | X |
| 2 | (NeuAc)3(Gal)4(GlcNAc)4(Man)3(GlcNAc)2(Fuc)1 | 4226.099 | (O) | (O) | (X) | (O) | (X) |
| 3 | (NeuAc)4(Gal)4(GlcNAc)4(Man)3(GlcNAc)2 | 4413.183 | (O) | (O) | (X) | (O) | (X) |
| 4 | (NeuAc)4(Gal)4(GlcNAc)4(Man)3(GlcNAc)2(Fuc)1 | 4587.273 | (O) | (O) | (X) | (O) | (X) |