FIGURE 3.

Effect of the R256H mutation on sensitivity to cytoskeletal disassembly. A, sterile filtered discs (0.5 cm in diameter) were presoaked in either 2 μl of dimethyl sulfoxide (control) or 2 μl of 0.1, 0.5, or 1 mm latrunculin A and placed on YPD plates containing 100 μl of evenly spread wild type or mutant cells (A₆₀₀ = 0.1). The plates were incubated at 30 °C for 48 h. B, quantification of growth inhibition by latrunculin A. The bar height indicates the area of growth inhibition (cm²) for the four concentrations of latruncilin A (mm) with error bars denoting the S.D. The area of growth inhibition for R256H cells was 1.9-fold higher for the highest latrunculin A concentration. Differences were statistically significant compared with wild type actin for all concentrations (p < 0.05).