FIGURE 7.

Effect of late addition of Bni1 on actin polymerization kinetics. A, polymerization of 10% pyrene-labeled wild type and R256H actin was fluorescently monitored over time as in Fig. 6 with the exception that 100 nm Bni was added to the reaction midway during elongation. Time of Bni addition is marked by an arrow. Concentrations for wild type and R256H actin were 1.5 and 2.25 μm, respectively. Shown is a representative plot from experiments performed at least three times with three independent actin preparations. A.U., arbitrary units.