FIGURE 8.

Actin polymerization kinetics in the presence of phalloidin. Polymerization of 10% pyrene-labeled actin was quantified by change in fluorescence over time with and without phalloidin and with and without Bni. Actin concentrations were 2.25 μm as was the concentration of phalloidin when included. 100 nm of the FH1-FH2 fragment of Bni1 was used when included. A, wild type actin. B, R256H mutant actin. Shown are representative plots from experiments performed at least two times with two independent actin preparations. A.U., arbitrary units.