FIGURE 9.

Polymerization kinetics of admixtures of wild type and mutant actin. A, ratios of wild type and R256H mutant actin were combined with a final concentration of 2.25 μm and induced to polymerize. The polymerization-dependent increase in fluorescence of the 10% pyrene-labeled actin was monitored over time. B, as in A, polymerization kinetics of ratios of wild type and R256H mutant actin was quantified in the presence 100 nm of Bni1. Shown are representative plots of experiments performed at least three times with three independent actin preparations. A.U., arbitrary units.