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Formation of Thr-AMP, Ser-AMP, and AMP by WT MST1. A, hydrolysis of [α-32P]ATP by MST1 (9 μm) in the presence of either Thr or Ser (20 mm). B and C, product formation was quantified over time. The final results were the average of three measurements with S.D. (error bars) indicated.
