TABLE 1.
Concentration of natural (natZn) and “exogenous” zinc (68Zn) found in zinc binding species (i.e., MTs and other than MTs) in the cytosolic fraction of cells under control conditions (no treatment) and after exposure of cultured HCEsv cells to either (i) 68ZnSO4 (100 μm), (ii) IL1α (100 units/ml), or (iii) a combination of 68ZnSO4 (100 μm) and IL1α (100 units/ml) for 24 h
The concentration of total zinc in all “zinc binding species” reflects the sum of concentrations of zinc (natZn + 68Zn) bound to MTs and to other species distinct to MTs. The concentration of zinc in zinc binding species was determined by IPD-HLPC-ICP-MS.
| Treatment | Zinc binding species | natZn | 68Zn | Total zinc (natZn+68Zn) |
|---|---|---|---|---|
| μm | μm | μm | ||
| Control | MTs | 1.5 ± 0.2 | 0.0 ± 0.0 | 1.5 ± 0.2 |
| Other than MTs | 2.2 ± 0.1 | 0.0 ± 0.0 | 2.2 ± 0.1 | |
| All zinc binding species | 3.7 ± 0.4 | 0.0 ± 0.0 | 3.7 ± 0.4 | |
| +68ZnSO4 | MTs | 0.48 ± 0.02 | 6.25 ± 0.04 | 6.7 ± 0.1 |
| Other than MTs | 2.2 ± 0.2 | 1.1 ± 0.1 | 3.3 ± 0.3 | |
| All zinc binding species | 2.7 ± 0.2 | 7.3 ± 0.2 | 10.0 ± 0.4 | |
| +68ZnSO4 + IL1α | MTs | 0.48 ± 0.04 | 7.77 ± 0.06 | 8.2 ± 0.1 |
| Other than MTs | 1.7 ± 0.3 | 1.30 ± 0.09 | 3.0 ± 0.5 | |
| All zinc binding species | 2.2 ± 0.3 | 9.07 ± 0.03 | 11.3 ± 0.3 | |
| +IL1α | MTs | 3.15 ± 0.02 | 0.0 ± 0.0 | 3.15 ± 0.02 |
| Other than MTs | 2.4 ± 0.3 | 0.0 ± 0.0 | 2.4 ± 0.3 | |
| All zinc binding species | 5.5 ± 0.2 | 0.0 ± 0.0 | 5.5 ± 0.2 |