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The apparent Km for UDP-GalNAc incorporation by β4GalNAc-T3-F and β4GalNAc-T4-F. β4GalNAc-T3-F and β4GalNAc-T4-F were incubated with increasing amounts of UDP-GalNAc for 15 min, and the amount of GalNAc incorporated into GLucα(PLRSKK)CA1–19 was determined. Nonlinear regression analysis assuming a single binding site for UDP-GalNAc provided the best fit and yielded an apparent Km of 1.4 μm for β4GalNAc-T3-F and 10.3 μm for β4GalNAc-T4-F. Error bars indicate S.E. LU, light units.
