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. 2012 Jul 6;287(34):29114–29124. doi: 10.1074/jbc.M112.347799

FIGURE 1.

FIGURE 1.

TANK is induced by RANKL. A, MDMs from wild-type mice were treated with M-CSF plus 25 ng/ml RANKL for 0, 6, 12, 24, 48, and 72 h. The mRNA levels of TANK and osteoclastogenic markers such as TRAP, Calcitonin receptor (Calcitonin R), and c-Fos were measured by quantitative PCR. Error bars: S.E. (n = 3). *, p < 0.05, **, p < 0.01, versus 0 h. B, MDMs from wild-type and Tank−/− mice were treated with M-CSF alone (0 h) or 50 ng/ml RANKL plus M-CSF (24 and 72 h) and the levels of TANK expression were analyzed by Western blotting. The relative band intensities of wild-type MDMs were measured using ImageJ and normalized by actin expression. C, MDMs from wild-type and Tank−/− mice were treated with M-CSF alone (M-CSF), 50 ng/ml LPS plus M-CSF (LPS), or 50 ng/ml TNF-α plus M-CSF for 24 h and the levels of TANK expression was analyzed by Western blotting. The data shown are representative of three independent experiments.