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. 2012 Jul 6;287(34):29114–29124. doi: 10.1074/jbc.M112.347799

FIGURE 7.

FIGURE 7.

Wild-type mice engrafted with Tank−/− bone marrow exhibit severe bone loss. A, expression levels of TANK in splenocytes from wild-type mice engrafted with wild-type marrow (+/+ → WT) and Tank−/− marrow (−/− → WT) specimens were measured by quantitative PCR. B, MDMs from +/+ → WT and −/− → WT specimens were treated with 50 ng/ml RANKL for 3 days and the numbers of osteoclasts were counted. C, representative images of distal femurs from +/+ → WT and −/− → WT specimens. D, TANK protein expression levels in bone marrow cells from +/+ → WT and −/− → WT mice. E, bone morphometric analyses of distal femurs from +/+ → WT and −/− → WT specimens using μCT. F, representative photographs of the metaphyseal portion of tibias from +/+ → WT and −/− → WT specimens (Villanueva bone staining). G and H, bone histomorphometric analyses of tibias from +/+ → WT and −/− → WT specimens. I, cortical bone thickness in the portion of cross section from proximal one-third of femur. Error bars: S.E. (n = 3). *, p < 0.05. J, bone histomorphometric analyses of the endosteum portion of cross section from proximal one-third of femur. Error bars: S.E. (n = 3). *, p < 0.05. K, serum concentrations of TRACP5b and ALP in +/+ → WT and −/− → WT mice. Error bars: S.E. (n = 3). *, p < 0.05. BV/TV, bone volume per tissue volume; Tb.Th, trabecular bone thickness; Tb.N, trabecular bone number; Tb.Sp, trabecular bone spacing; Oc.N/BS, osteoclast number per bone surface; Ob.N/BS, osteoblast number per bone surface. Es.ES/Es.BS, endosteum eroded surface per endosteum bone surface; Es.BFR/Es.BS, endosteum bone formation rate per endosteum bone surface.