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. 2012 Jun 1;3(1):16. doi: 10.1186/2049-1891-3-16

Table 1.

Primer sequences for the target genes

Target genes GenBank accession numbers PCR products (bp) Primer sequences
β-actin
NM 205518
300
F: 5′- tgcgtgacatcaaggagaag -3′
      R: 5′- tgccagggtacattgtggta -3′
LEPR
NM 204323
87
F: 5′- gcatctctgcatctcaggaaaga -3′
      R: 5′- gcaggctacaaactaacagatcca -3′
SREBP-1c
NM 204126
104
F: 5′- gcagaagagcaagtccctcaa -3′
      R: 5′- tcggcatctccatcacctc -3′
SREBP-2
XM 416222
108
F: 5′- cccagaacagcaagcaagg -3′
      R: 5′- gcgaggacaggaaagagagtg -3′
HMGCR
AB 109635
137
F: 5’-ttggatagagggaagagggaag -3’
      R: 5’- ccatagcagaacccaccaga-3’
CYP7A1
AB 109636
106
F:5’- cattctgttgccaggtgatgtt -3’
      R:5’- gctctctctgtttcccgcttt -3’