Table 1.
Relative quantitative real-time PCR primers and probes
| EST | Primers | Taqman Probe |
|---|---|---|
| TC293 |
FW 5’ AACATCTACAGCAAGTTTGACACC 3’ |
5’ FAM- AGGTGACCGCCTGGATACTCCGCA-TAMRA 3’ |
| RV 5’ CCCGTCGATGCAGGTTTTGG 3' | ||
| BEAI101TR |
FW 5’ CGGAAGAAACGAGAAATACGAGAC 3’ |
5’ FAM- TGCGTCGGCACACACTGGTACAGC-TAMRA 3’ |
| RV 5’ TACATGAGAACAGTAGCATATAGGG 3’ | ||
| TC12256 |
FW 5’ CTTCACATTCAACACGCCCTAC 3’ |
5’ FAM-AGCCACAGCAACGCCATCGCCG-TAMRA 3’ |
| RV 5’ AAAACCGCTACGGCAAATGC 3’ | ||
| TC14244 |
FW 5’ TTAAACATTCTTTCGCTCATCAGTC 3’ |
5’ FAM-CCGCACGACGCAAGCCGAAA-TAMRA 3’ |
| RV 5’ TACATGAGAACAGTAGCATATAGGG 3’ | ||
| 18S |
FW 5’ CCTGAGAAACGGCTACCACATC 3’ |
5’ FAM-AGGAAGGCAGCAGGCGCGC-TAMRA 3’ |
| RV 5’ GTGCCGGGAGTGGGTAATT 3’ |
a Based on BmiGI Version 2 designations (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=b_microplus).
b Forward (FW) & reverse (RV).