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. 2012 Sep 7;7(9):e43900. doi: 10.1371/journal.pone.0043900

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© 2012 Tare et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Open complex was formed in the presence of 100 µM of iNTPs (+1 and +1, +2) and the transcription was initiated by adding heparin and all the four NTPs. The run-off transcripts were resolved on 8% urea–PAGE. The initial transcribed sequence (ITS) for all the promoters is shown. B The graph shows the quantification of transcripts formed in the absence and presence of +1 iNTP and +1, +2 iNTPs. The amount of run-off transcripts formed was measured by densitometry and indicated as AU on Y axis. C Effect of pppGpp on open complex. Open complex was formed in the presence of increasing concentrations of pppGpp as indicated and the transcription was initiated as described before. The run-off transcripts were resolved on 8% urea–PAGE. D The graph shows the quantification of transcripts formed in the presence of increasing concentrations of pppGpp. The amount of run-off transcripts formed was measured by densitometry and indicated as AU on Y axis.