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. 2012 Sep 7;7(9):e43722. doi: 10.1371/journal.pone.0043722

Figure 5. SDS-PAGE and CBB staining using purified FimA fimbriae.

Figure 5

Purified FimA fimbriae were denatured in an SDS-containing buffer with 2-mercaptoethanol by heating at 60 to 100°C for 10 min, then subjected to SDS-PAGE and CBB staining. Samples were as follows: purified from P. gingivalis ATCC 33277 Δmfa1 (native 33277 FimA fimbriae, lane 1), P. gingivalis ATCC 33277 Δmfa1 Δfim cluster with fimA of ATCC 33277 (I) (lane 2), TDC60 (II) (lane 3), 6/26 (III) (lane 4), W83 (IV) (lane 5), HG564 (IV) (lane 6), and HNA99 (V) (lane 7) introduced. Note that CBB staining did not visualize a ladder band as seen in immunoblot analysis in Fig. 2.