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. 2012 Sep 7;7(9):e43722. doi: 10.1371/journal.pone.0043722

Figure 6. ELISA using absorbed antisera and whole-cell sonicates as antigen.

Figure 6

Whole-cell sonicates were coated on ELISA plates as antigens. Antisera from mice immunized with each pure genotype fimbriae were used after absorption with the fimbria-deficient mutant 33277 Δmfa1 Δfim cluster. “Non” indicates non-immunized mouse sera. W83 rarely produces FimA protein and fimbriae. Data show mean ± SD. Asterisks indicate statistical significance compared with Non (* p<0.05, ** p<0.01). Note that scales of Y axes are adjusted in order to compare titers clearly.