Table 1. Summary of serological analyses.
Antisera against | Experiment* | Antigen | ||||||
33277** | TDC60 | 6/26 | W83 | HG564 | HNA99 | |||
Native 33277 (I) | ELISA | Cell | +++ | − | ++ | − | − | − |
Pure | +++ | − | − | − | − | − | ||
IB | Poly | ++ | − | − | − | − | − | |
Mono | + | − | + | − | − | − | ||
33277 (I) | ELISA | Cell | +++ | − | − | − | − | − |
Pure | +++ | − | − | − | − | − | ||
IB | Poly | +++ | − | − | − | − | − | |
Mono | + | − | − | − | − | − | ||
TDC60 (II) | ELISA | Cell | + | +++ | ++ | − | − | − |
Pure | ++ | +++ | ++ | − | − | − | ||
IB | Poly | ++ | + | ++ | − | − | − | |
Mono | + | + | + | − | − | − | ||
6/26 (III) | ELISA | Cell | + | − | +++ | − | − | − |
Pure | − | − | +++ | − | − | − | ||
IB | Poly | + | + | +++ | − | − | − | |
Mono | − | − | + | − | − | − | ||
W83*** (IV) | ELISA | Cell | − | − | ++ | − | +++ | ++ |
Pure | − | − | + | +++ | +++ | − | ||
IB | Poly | + | − | + | − | ++ | − | |
Mono | − | − | − | − | + | − | ||
HG564 (IV) | ELISA | Cell | − | − | − | − | +++ | − |
Pure | − | − | − | +++ | +++ | − | ||
IB | Poly | − | − | − | − | +++ | − | |
Mono | − | − | − | − | + | − | ||
HNA99 (V) | ELISA | Cell | − | − | − | − | + | +++ |
Pure | − | − | − | − | − | +++ | ||
IB | Poly | − | − | − | − | ++ | ++ | |
Mono | − | − | − | − | − | + |
Experiment; ELISA/Cell, ELISA/Pure, IB/Poly and IB/Mono are from Figs. 6, 7, 8, and 9, respectively.
Antigen of 33277 in ELISA/Pure is 33277 FimA fimbriae, not native 33277.
W83 rarely produces FimA protein and fimbriae.
IB, immunoblot; Cell, whole-cell sonicates; Pure, purified FimA fimbriae; Poly, partially dissociated FimA fimbriae; Mono, FimA monomer.
+++ reacted strongly; ++ reacted moderately; + reacted weakly; − did not react.