Table 2.
Comparison of the optimal knockout strategies for succinate production for the full EM set and different EM samples
| Test | No Total EMs (suc) | Reaction knockouts | No EMs (suc) | Est. rate |
|---|---|---|---|---|
| Full EM set | 100 273 (48 602) | ACALD, G6PDH2r, GLUDy, LDH_D, NADTRHD, PFL, PYK, SUCDi | 406 (320) | 6.897 |
| Sample 1 | 8745 (3962) | ACALD, GLUSy, ICL, LDH_D, PFL, PGI, SUCDi, THD2 | 59 (34) | 3.397 |
| Sample 2 | 9001 (3979) | ACALD, GLUSy, ICL, LDH_D, ME2, PFL, PGI, SUCDi | 69 (30) | 3.397 |
| Sample 3 | 8607 (4011) | ACALD, ATPS4r, GLUDy, ME1, PFL, PYK, SUCDi, TKT2 | 50 (37) | 4.435 |
| Sample 4 | 8682 (3838) | ACALD, ACKr, GLUDy, ICL, LDH_D, PFL, RPE, SUCDi | 48 (42) | 6.647 |
| Sample 5 | 8489 (3553) | ACALD, ACKr, GLUSy, LDH_D, PFL, PGI, SUCDi, THD2 | 76 (52) | 3.473 |
| Sample 6 | 8453 (3574) | ATPS4r, GLUSy, ME1, NADTRHD, PFL, PYK, SUCDi, TALA | 44 (36) | 2.004 |
| Sample 7 | 9056 (4080) | ACALD, GLUSy, ME1, ME2, PFL, PGI, SUCDi, THD2 | 81 (55) | 3.473 |
| Sample 8 | 8877 (4228) | ACALD, ATPS4r, FBP, G6PDH2r, GLUSy, ME1, NADTRHD, PFL | 38 (10) | 0.000 |
| Sample 9 | 8647 (4007) | ACALD, G6PDH2r, GLUSy, ME2, NADTRHD, PFL, PYK, SUCDi | 41 (30) | 6.899 |
| Sample 10 | 9097 (4129) | ACALD, ATPS4r, GLUSy, LDH_D, NADTRHD, PFL, SUCDi, TALA | 41 (31) | 0.207 |
Total number of EMs (succinate producing); Optimal reaction knockouts; Number of remaining EMs (succinate producing); Estimated production rate (mmol/gDW/h) computed from MOMA.