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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Dig Liver Dis. 2012 Jun 19;44(10):819–826. doi: 10.1016/j.dld.2012.05.013

Figure 6. Production of DSS-induced proinflammatory cytokines is attenuated upon treatment with A2BAR siRNA-loaded NPs.

Figure 6

After 8 days of DSS treatment, mice were sacrificed and their colons were collected for isolation of RNA. Proinflammatory cytokine and chemokine mRNA levels were determined by real-time PCR. 36B4 was used as an internal control (A). Protein levels of proinflammatory cytokines were measured in supernatant collected from colonic organ cultures after 24 hours of incubation at 37°C. Supernatants were centrifuged and the cytokine and chemokine levels were measured using a MULTI-SPOT plate (B). 6 mice were used for each experimental group and error bars represent standard deviation. Statistical significance was assessed using Students t-test, where **, p <.01 and ***, p < .001.