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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 22;189(6):2985–2994. doi: 10.4049/jimmunol.1200846

Figure 3. The altered tumor microenvironment by C5a.

Figure 3

(A) Total tumor samples were collected and RNAs were extracted. qRT-PCR data revealed the downregulation of VEGF, arginase, and TNF-α mRNA levels in C5a expressing tumors. *p<0.05, **p<0.01. (B) Sorted CD11b+ innate immune cells and CD11b− tumor cells were performed for qRT-PCR analysis. Data indicate that VEGF mRNA level is significantly decreased in C5a expressing tumor cells while the CD11b+ cells sorted from SKOV-3 C5a have significantly lower levels of iNOS mRNA. *p<0.05 (C) The SKOV-3 C5a infiltrating F4/80+ macrophages expressed significantly lower levels of arginase mRNA. *p<0.05.