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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 22;189(6):2985–2994. doi: 10.4049/jimmunol.1200846

Figure 6. Low C5a-expressing lymphoma cells have significantly decreased tumor progression.

Figure 6

(A) C5a levels of RMA cells transfected with C5a or CV. Data indicate that RMA 3CF4 clone secreted higher level of C5a than RMA 1474 clone. (B) Wildtype C57Bl/6 mice were injected with low C5a-expressing lymphoma RMA-1474 cells (n=10) or RMA CVA1 cells (n=15) and tumor growth was recorded. Data are shown as mean±s.e.m. *p<0.05. (C) Cells from spleen and tumor from tumor-bearing mice were stained with Gr-1 and CD11b. Representative dot plots and summarized data are shown. (D) Cells were stimulated with PMA/ionomycin and surface stained with CD8 and IFN-γ intracellularly. Representative dot plots (cells were gated on the CD8+ T cells), summarized IFN-γ-producing CD8+ T cells, and total CD8+ T cells are shown. (D) Cells were stimulated with PMA/ionomycin and surface stained with CD4 and IFN-γ intracellularly. Representative dot plots (cells were gated on the CD4+ T cells), summarized IFN-γ-producing CD4+ T cells, and total CD4+ T cells are shown.