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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 22;189(6):2985–2994. doi: 10.4049/jimmunol.1200846

Figure 7. C5a regulates CD4 T cell differentiation.

Figure 7

Peritoneal macrophages were stimulated with varying concentrations of C5a (0–500 ng/ml) for 24 h and then co-cultured with naïve CD4 OVA Tg T cells in the presence of OVA for 3 days. Cells were then stained intracellularly with IFN-γ (A) and IL-17A (B). For Treg induction assay, macrophages were co-cultured with naïve CD4 OVA Tg T cells in the presence of OVA with varying concentrations of C5a (0–500 ng/ml) for 4 days. Cells were stained intracellularly with Foxp3 (C). Representative dot plots and summarized data are shown. Cells were gated on the CD4+ T cells. Data are representative of at least three independent experiments.