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. Author manuscript; available in PMC: 2013 Sep 15.

Published in final edited form as: Free Radic Biol Med. 2012 Jul 20;53(6):1346–1357. doi: 10.1016/j.freeradbiomed.2012.07.005

Fig. 7 — HepG2 E47 cells and C34 cells were treated with 100 mM ethanol in the absence or presence of 2.5 mM 3MA or 0.2 µg/ml rapamycin for 8 days. Cell viability was determined by MTT assay (A). *, P<0.05 compared with ethanol group; cell morphology under the light microscope (B); Oil Red O Staining in E47 (C) or C34(D) cells; GSH (E); LC3II/LC3I ratio (F). *, P<0.05 compared to control; **, P<0.05 compared to ethanol alone. N=3.

Fig. 7 — HepG2 E47 cells and C34 cells were treated with 100 mM ethanol in the absence or presence of 2.5 mM 3MA or 0.2 µg/ml rapamycin for 8 days. Cell viability was determined by MTT assay (A). *, P<0.05 compared with ethanol group; cell morphology under the light microscope (B); Oil Red O Staining in E47 (C) or C34(D) cells; GSH (E); LC3II/LC3I ratio (F). *, P<0.05 compared to control; **, P<0.05 compared to ethanol alone. N=3.