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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 15;189(6):2727–2734. doi: 10.4049/jimmunol.1200380

Figure 8. 3BP2 is required for normal huMC cytokine production.

Figure 8

CD34+ peripheral blood-derived huMCs with normal or reduced levels of 3BP2 (NT shRNA and 3BP2 shRNA respectively) were sensitized O/N with biotinylated IgE and stimulated for 6 h with 1 µg/ml streptavidin (SA). IL-8 and GM-CSF production were measured by ELISA (A and B) and IL-8 transcripts relative to a control RNA were measured by real time PCR (C). Results are expressed as the mean +/− SD. Significant (***p<0.0001) difference was found between NT shRNA and 3BP2 shRNA. Data are the mean of triplicates and it is representative of 3 independent experiments from 3 different donors. N.D. means not detectable.