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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 17;189(6):2918–2930. doi: 10.4049/jimmunol.1200065

FIGURE 3.

FIGURE 3

Contribution of the CD5-CK2 signaling pathway to the generation of Th1, Th2, and Th17 cells from naïve CD4+ T-cells. Naïve CD4+ T-cells were co-cultured with irradiated APCs and stimulated with MOG35-55 peptide (A) or anti-CD3 (B) under non-polarizing (unpolarized) or polarizing conditions and generation of Th subpopulations was evaluated. Numbers in each quadrant represent the average frequency of CD4+ T-cells expressing IFNγ, IL-17a and/or IL-4±SEM from four independent experiments performed with at least three mice per experiment. C, Percent 7-AAD+CD4+ T-cell at 24 h and 120 h and D, RORγt+CD4+ T-cells at 120 h following stimulation with MOG35-55 peptide under Th17 polarizing conditions. Data is mean±SEM of three independent experiments with at least two mice/group. E, pSTAT1 (Ser727) and F, pSTAT3 (Tyr705) in CD4+ T-cells stimulated for 72h with MOG35-55 peptide under Th17 polarizing conditions. The filled shaded histograms are unstimulated and the dashed histograms are isotype immunoglobulin stained cell controls. For pSTAT1 (E), the MFI of 2D2.CD5ΔCK2BD is 52±4.8 and 2D2.CD5WT is 30±4.3. For pSTAT3 (F) the MFI for 2D2.CD5ΔCK2BD is 32±2.9 and 2D2.CD5WT is 21±3.5. Histograms represent data from one representative mouse per group from two mice/experiment, n=3 experiments. *p<0.05, **p<0.01, ***p<0.001.