Table III.

CD5-CK2 signaling pathway regulates TCR-induced apoptosis in T-cells

Anti- CD3 (μs/ml)	%Annexin V+7-AAD−			MOG35-55 (μg/ml)	%Annexin V+7-AAD−
	CD5WT	CD5KO	CD5ΔCK2BD		2D2.CD5WT	2D2.CD5KO	2D2.CD5 ACK2BD
0	9.7±1.3	10.7±1.8	9.8±0.3	0	15.0±1.1	27.0±3.6	26.2±2.8b
0.1	9.6±0.7	10.9±0.1	9.5±0.7	1	19.6±0.6	39.7±2.1c,d	35.9±1.5c
1	8.8±0.6a	14.0±1.3	9.6±0.7a	10	33.1±0.3e	43.1±2.6f	46.9±7.6
				100	31.4±0.5g	39.0±2.2g,h	71.4±4.4

Cells were stimulated with varying concentrations of anti-CD3 or MOG_35-55 peptide in the presence of irradiated APCs for 24 h. Apoptosis was evaluated by staining cells with Annexin V and 7-AAD.

Data are presented as % of events ± SEM.

^ap<0.01; vs. CD5KO mice

^bp<0.05; 2D2.CD5ΔCK2BD vs. 2D2.CD5WT mice

^cp<0.001; vs. 2D2.CD5WT mice

^dp<0.05; 2D2.CD5KO mice vs. 2D2.CD5ΔCK2BD mice

^ep<0.01; 2D2.CD5WT mice vs. 2D2.CD5ΔCK2BD mice

^fp<0.05; 2D2.CD5KO mice vs. 2D2.CD5ΔCK2BD mice

^gp<0.001; vs. 2D2.CD5CK2BD mice

^hp<0.01; 2D2.CD5KO mice vs. 2D2.CD5WT mice