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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 6;189(6):2824–2832. doi: 10.4049/jimmunol.1201114

Figure 2. Stable knockdown of GRK2 and GRK6 expression in RBL-2H3 cells stably expressing CXCR1 and CXCR2.

Figure 2

A & B) A representative histogram of FACS analysis showing surface expression of CXCR1 (A) and CXCR2 (B) in RBL-2H3 cells after staining with CXCR1 or CXCR2 specific antibodies. B) RBL-2H3 cells expressing CXCR1 (left panel) or CXCR2 (right panel) were transfected with scrambled shRNA control lentivirus (mock) or shRNA lentivirus specific for GRK2 or GRK6 knockdown. Puromycin resistant cells were selected and single clones were generated and analyzed by immunoblotting.