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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: Free Radic Biol Med. 2012 Jul 14;53(6):1213–1221. doi: 10.1016/j.freeradbiomed.2012.07.003

Figure 1. High glucose decreases blood-brain barrier integrity.

Figure 1

A: Confluent monolayers of human brain microvascular endothelial cells (HBMECs) were cultured on Transwell filters and the diffusion of FITC-conjugated dextran (150 kDa; 100μg/ml) in 5 mmol/l D-glucose (normal glucose, NG), 25 mmol/l D-glucose (HG), or 5 mmol/l D-glucose plus 20 mmol/l L-glucose (LG) was measured at various time-points. Data are expressed as the mean fluorescence intensity ± SD of three individual Transwell filters where 100% corresponds to 6.7±0.5μg/ml FITC dextran in NG for 1 week. The graph is a representative example of results from three independent experiments. B: Trans-endothelial electrical resistance was measured in real-time in confluent monolayers of HBMECs. Data are expressed as percentage of the mean resistance ± SD of three independent experiments performed in triplicate; 100% corresponds to 143 ± 19.6 Ω·cm2 in NG for 1 week.