Table 1.
Primary antibodies used in double immunofluorescence studies
| Antigen | Clone/code | Source | Company | Dilution |
|---|---|---|---|---|
| 19S regulator | AH1.1 | Mouse (mAb) | Abcam | 1/100 |
| 20S subunits | PA1-979 | Rabbit (pAb) | Thermo Scientific | 1/20 |
| Atrogin-1 (Fbx32) | ab74023 | Rabbit (pAb) | Abcam | 1/100 |
| BAG3 | ab47124 | Rabbit (pAb) | Abcam | 1/2000 |
| CHIP | PC711 | Rabbit (pAb) | Calbiochem | 1/1000 |
| Filamin C | FLNC d16-20 | Rabbit (pAb) | Custom-made (BioGenes) | 1/1000 |
| HDAC6 | BV-48 | Rabbit (pAb) | BioVision | 1/50 |
| Hsp20 | HSP20-11 | Mouse (mAb) | BioTrend | 1/100 |
| Hsp22 | 520817 | Mouse (mAb) | R+D Systems | 1/50 |
| Hsp27 | 2B4 | Mouse (mAb) | Novocastra | 1/500 |
| Hsp40 | C64B4 | Rabbit (pAb) | Cell Signalling | 1/50 |
| Hsp60 | D307 | Rabbit (pAb) | Cell Signalling | 1/50 |
| Hsp70 | 4872 | Rabbit (pAb) | Cell Signalling | 1/50 |
| Hsp90 | E289 | Rabbit (pAb) | Cell Signalling | 1/25 |
| LAMP2 | H4B4 | Mouse (mAb) | DSHB | 1/10 |
| LC3 | 2775 | Rabbit (pAb) | Cell Signalling | 1/200 |
| Myotilin | RS034 | Mouse (mAb) | Novocastra | 1/20 |
| p53 | PAb 240 | Mouse (mAb) | Abcam | 1/250 |
| p62 | GP62-N | Guinea pig (pAb) | Progen | 1/100 |
| Ubiquitin | ab7780 | Rabbit (pAb) | Abcam | 1/50 |
| Ubiquitin +1 | 40B3 | Mouse (mAb) | Abcam | 1/25 |
| VCP | 5 | Mouse (mAb) | ABR | 1/500 |
mAb = monoclonal antibodies; pAb = polyclonal antibodies.