Total GSH contents in mtCCs after AFN or DNCB treatment and in RA or hyperoxia-exposed mtCCs after AFN or SFN treatment. (A) Total GSH contents were greater in AFN- or DNCB-treated mtCCs 5 h after treatment than in vehicle-treated controls. Analyses indicated the independent effects of and an interaction between treatment and time on GSH levels. Data (mean±SEM, n=6) were analyzed by two-way ANOVA with Bonferroni post hoc with significance noted at p<0.05 (*different than same time/vehicle; %different than same time/AFN; $different than same treatment/0 h; #different than same treatment/2 h). (B, C) mtCCs were treated with 1 μM AFN or 5 μM SFN for 1 h, as described in Methods and exposed to RA or hyperoxia for (B) 5 h or (C) 24 h. Analyses of total GSH levels indicated an independent effect of AFN and SFN treatment at 5 h and the effects of treatment and exposure at 24 h. Data (mean±SEM, n=4–6) were analyzed by two-way ANOVA with Bonferroni post hoc with significance noted at p<0.05 (*different than vehicle/21% O2; $different than vehicle/85% O2; #different than same treatment/21% O2). GSH, glutathione; RA, room air; SFN, sulforaphane.