Table 1.
Residues interchanged to generate the RebC-10x and StaC-10x constructs (see Figure S1 for a complete sequence alignment).
| RebC | StaC | Function |
|---|---|---|
| Glu36 | Asp37 | FAD binding (adenine) |
| Gln37 | Ala38 | FAD binding (adenine) |
| Thr38 | Gly39 | FAD binding (adenine) |
| Gln117 | Ala118 | FAD binding (phosphate) |
| Arg46 | Lys47 | FAD binding (isoalloxazine) |
| Gly48 | Ser49 | FAD binding (isoalloxazine) |
| Phe216 | Val221 | Substrate binding pocket |
| Ala231 | Ser236 | Substrate binding pocket |
| Arg239 | Asn244 | FAD (isoalloxazine) & substrate binding |
| Thr241 | Val246 | Substrate binding pocket |