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. 2012 May 14;5:234. doi: 10.1186/1756-0500-5-234

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Copyright ©2012 Zhang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Specificity of the slot blot assay using MBD1 protein. (a) Methylation status of the λDNA assessed by HpaII endonuclease restriction analysis shows that in vitro methylated λDNA (M+) was resistant to endonuclease cleavage, but unmethylated λDNA (M-) was not. (b) Methylated λDNA (100%) was added in increasing amounts to unmethylated λDNA (0%) to create a dilution series of methylated DNA from 0% to 100% in a total amount of 500 ng. The blot was stained with methylene blue to ensure the equal loading of total DNA. (c) The same blot detected by MBD1. (d) The observed band intensity was plotted against the methylation percentage. The regression equation with an adjusted R² of 0.9705 was presented on figure.