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Immunofluorescence labeling of unfixed porcine cartilage sections following heparinase III. Dual IF labeling of (A) HS epitope 3G10 and (B) perlecan exhibited a nearly one-to-one overlay (C). (D) 3G10 and (E) type VI collagen co-localized in the pericellular space around cell-voids (F). Heparinase III digestion had no effect on labeling of (G) perlecan or (H) type VI collagen and did not disrupt their co-localization in the PCM (I). Scale bar = 20 μm.
