Table 1.
Summary of transformation studies in Coffea sp∗.
| DNA delivery method | Coffea species | Explants used | Binary vector | Agrobacterium strain | Promoter | Selection marker | Target gene | Results | Reference | Country |
|---|---|---|---|---|---|---|---|---|---|---|
| Direct delivery electroporation | C. arabica | Protoplast | NA | — | pGA472 | nptII | uidA | TE | [15] | USA |
| C. arabica | SE | pCAMBIA 3201 | — | CaMV35S | bar | uidA | TGI | [16] | Venezuela | |
| C. canephora | Endosperm | pCAMBIA 1301 | — | CaMV35S | hpt | uidA | TE | [17] | India | |
|
| ||||||||||
| Microprojectile bombardment | C. arabica | Leaf | pPIGK | — | EF-1a | bar | uidA | TE | [18] | France |
| C. arabica | ET | pCAMBIA 2301 | — | CaMV35S | nptII | uidA | TGI | [19] | Colombia | |
| C. arabica | ET | pBI-426 | — | CaMV35S | nptII | uidA | TGI, PR | [20] | Brazil | |
| C. canephora | ET | pCAMBIA 3301 | — | CaMV35S | bar | uidA | TGI, PR | [21] | Brazil | |
| C. arabica | SE | pCAMBIA 2301 | — | CaMV35S | nptII | uidA | TE | [22] | Costa Rica | |
| C. arabica | ET | pBI-426 | — | CaMV35S | nptII | uidA | TGI, PR | [23] | Brazil | |
|
| ||||||||||
| Indirect delivery A. tumefaciens | C. arabica | Protoplast | pGV2260 | NA | CaMV35S | hpt | uidA | TE | [24] | France |
| C. canephora | ET | pIGI121-Hm | EHA101 | hpt | uidA | TGI, PR | [25] | Japan | ||
| C. arabica and C. canephora | SE | pBIN19 | LBA4404 | EF-1α | csr-1-1 | cry1Ac | TGI, PR | [26] | France | |
| C. canephora | ZE, SE, H | pBECKS 400 | EHA101 | CaMV35S | hpt | uidA | TGI, | [27] | India | |
| C. arabica and C. canephora | ET, SE | pHIBI –IG | EHA101 | CaMV35S | hpt | gfp & CaMXMTI | RNAi, PR | [28] | Japan | |
| C. canephora | H | pBECKS 400 | EHA 101 | CaMV35S | hpt | uidA | TGI, PR | [29] | India | |
| C. canephora | ET | pCAMBIA 3301 | EHA105 | CaMV35S | ppt | uidA | TGI | [30] | Brazil | |
| C. canephora | ET, Leaf | pER10W-35SRed | C58 | CaMV35S | nptII | DsRFP | TGI, PR | [31] | Mexico | |
| C. canephora | SE | pCAMBIA 1381 | A4, EHA 101 | CaMV35S | hpt | NMT | RNAi | [32] | India | |
| C. canephora | SE | pBIN19 | LBA 4404 | EF-1 | csr-1-1 | cry1Ac | Field test | [33] | France | |
| C. arabica | ET | pCAMBIA 3300 | EHA 105 | CaMV35S | bar | ACC-oxidase | AE | [34] | Brazil | |
| C. canephora | ET | pCAMBIA 3301 | EHA 105 | CaMV35S | bar | uidA | TGI, PR | [35] | Brazil | |
| C. canephora | Embryos | pER10W-35SRed | C58C1 | CaMV35S | WUSCHEL | DsRFP | TGI,PR | [36] | Mexico | |
| C. canephora and C. arabica | ET | pBECKS 2000 | EHA 101, EHA 105, LBA 4404, AGL 1 | CaMV35S | hpt, visual | uidA, sgfp | TGI, PR | [37] | India | |
| C. arabica | ET | pMDC32 | LBA1119 | CaMV35S | hpt | sgfp | PR | [38] | France | |
|
| ||||||||||
| A. rhizozenes | C. arabica and C. canephora | SE | pBIN 19 | A4 | CaMV35S | hpt | uidA | TGI, PR | [39] | France |
| C. arabica | Leaf | NA | IFO 14554 | NA | NA | TGI, PR | [40] | Japan | ||
| C. canephora | SE | pBIN 19 | A4 | NA | csr-1-1 | cry1Ac | TGI, PR | [41] | France | |
| C. canephora | SE | pCAMBIA 1301 | A4 | CaMV35S | hpt | uidA | TGI, PR | [42] | India | |
| C. arabica | H | pBIN 19 | A4 | CaMV35S | visual | uidA | TGI, PR | [43] | France | |
| C. arabica | H | pCAMBIA 2300 | A4 | CaMV35S | visual | gfp | TGI, PR | [44] | France | |
| CaMV35S | ||||||||||
∗Updated from Etienne et al. [45]; ET: embryogenic tissue; SE: somatic embryos; H: hypocotyls; TE: transient gene expression; TGI: target gene integration; PR: plant rregeneration; RNAi: RNA interference, AE: antisense expression.