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Autofluorescence blocked with Sudan Black B staining of testis cells in situ and in vitro. Piglet testis tissue sections (a, b) and dissociated cells (c, d) were stained with Sudan Black B, examined under a brightfield microscope (a, c) and a confocal laser scanning microscope, excited with a 405 nm laser with detection of emissions within 575–620 nm (b, d) (with brightfield overlay). Scale bars, 100 μm.
