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. 2012 Aug 29;2012:745185. doi: 10.5402/2012/745185

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Copyright © 2012 Cecilia Vasti et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Protein ubiquination in cardiomyocytes. Transverse ventricular sections of nontreated WT and KO and doxorubicin-treated WT and KO hearts (WTD, KOD) (n = 4) were immunnostained with antibodies against ubiquitin, revealed with biotin-coupled antiolyclonal rabbit and FITC-coupled streptavidin (green). The membrane was stained with wheat germ agglutinin (TRICT-coupled WGA, red) and nuclei with DAPI (blue). The images were scanned with fluorescent microscope (upper panel), original magnification 400x. Scale bar: 100 μm. Note ubiquitin-protein aggregates (arrows) and ubiquitin-labeled proteins adjacent to the membrane in erbB4-KOD. Detailed images of KOD sections with large ubiquitin-posistive protein aggregates were obtained by confocal microscopy (lower panel).