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Protocol used for song exposure and song-induced ZENK protein expression in NCM. a Top sonograms of the adult male zebra finch songs used in the song-exposure protocol. Bottom scheme of the song-exposure protocol. The numbers in the boxes correspond to the song presented (top), see “Methods” for details. b Song-induced ZENK expression in NCM slices prepared as for electrophysiology from a song-exposed (left) and a control (right) adult male zebra finch
